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Image May the Force be with you.

    "More midi-chlorians than me Anakin Skywalker has...". Was he really talking about mitochondria, our  "sub-microscopical endosymbiots" living in cells? This section provides  a few protocols to measure the activity of mitochondrial enzymes.

It is split, rather arbitrary, in the following sub-sections (click to load):

Reagents:


Extraction protocols:

Enzymatic assays:

Functional assays:

  • ATP synthesis
  • ATP/ADP exchange
  • Respiration
  • Membrane Potential
  • pH gradient
  • Production of Reactive Oxygen Species
  • Ca2+ uptake and Ca2+ capacity
  • Swelling

Protein assays:

  • Protein measurement
  • Electrophoresis and Western Blot
  • Non-denaturing electrophoresis of mitochondrial enzyme complexes
  • 2D electrophoresis

to be continued...   

Last Updated ( Saturday, 11 July 2015 )
 
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