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Mitochondria Facts Print E-mail
Article Index
Heavy and Light Mitochondria
Tissue difference
Membrane Morphometry
Electron Flux
Structure and Function

Structural, dimensional, and functional characteristics of isolated liver mitochondria.


These data are excerpted from:
Adrian R.L.Gear and Jana M.Bednarek, “Direct counting and sizing of Mitochondria in solution”,
The Journal of Cell Biology (1972), 54, pp. 325–345.

1.Characterization of light and heavy liver mitochondrial fractions.
Heavy mitochondria.

ParameterSpecific content per mg proteinParticle content
(units per particle x 10-9 )
 Number of particles
3.95 x 109
-----
 Diameter by Coultier counter
1.02 micron
-----
 Diameter by electron microscopy
0.99 micron
-------
 Volume
0.55 micron3
-------
 DNA
0.155 microg
0.0392 microg
 RNA
9.0 microg
2.28 microg
 Cytochrome a+a3
0.49 nmol
0.124 nmol
 Cyt. c oxidase activity, k-1
45
11.4
 Total iron, nmol
4.1
1.04

Light mitochondria:

Parameter Specific content per mg protein Paritcle content
(units per particle x 10-9 )
 Number of particles
 5.14 x 109
----
 Diameter by Coultier counter
0.82 micron
 --- 
 Diameter by electron microscopy
0.72 micron
----
 Volume
0.3 micron3
---
 DNA
0.245 microg
0.0477 microg
 RNA
13.8 microg
2.68 microg
 Cytochrome a+a3
0.66 nmol
0.128 nmol
 Cyt. c oxidase activity, k-1
70
13.6
 Total iron, nmol
5.3
1.03

 


Last Updated ( Saturday, 12 May 2007 )
 

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